Whole body protein synthesis rates were estimated in 70 g rats using an intravenous injection of l u14c threonine l u14c lysi these studies were undertaken to investigate the influence of the precursor amino acid on the measurement of whole body protein turnover by the flooding dose method. F nce kino and okumura 1987 estimated nitrogen balance in 1421 day old chickens as 029 gday.
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Whole body protein synthesis measurement. Where whole body protein losses includes in proteins in eggs or molted feathers. Whole body protein metabolism was characterized by using the end product model based on nitrogen excretion and leucine catabolism. Introduction over recent years the mechanism of protein synthesis in animals has gradually been clarified. Nitrogen flux and rates of protein degradation and synthesis in the fed state were significantly lower at a dietary protein intake of 10 gkg 1d 1 in lactating women than in their nonlactating postpartum counterparts. Whole body protein synthesis and breakdown were determined using repeated oral administration of 15 n glycine for 6072 h and 15 n enrichment in urinary urea was measured. Replication initiator 1 repin1 is a zinc finger protein highly expressed in liver and adipose tissue and maps within a quantitative trait locus qtl for body weight and triglyceride tg levels in the rat.
Primarily via the stimulation of protein synthesis rates. If negative whole body protein net synthesis accretion is whole body protein net degradation. Rates of whole body protein synthesis and breakdown expressed as g proteinkg body weightday were significantly p less than 005 and positiviely correlated with per cent body surface area total burn per cent third degree burn and per cent open wound. The energy cost of whole body protein synthesis was calculated to be 535 kj per g protein synthesis and hence on a molar basis 752 atps are required per peptide bond synthesis. The qtl has further been supported as a susceptibility locus for dyslipidemia and related metabolic disorders in congenic and subcongenic rat strains. We utilized iaao to determine the maximal anabolic response to.
This dynamic relationship between protein synthesis and protein breakdown is generally considered to result in net protein balance in healthy weight stable adults ie protein synthesis is equivalent to breakdown where skeletal muscle stores turn over at a rate of 1 2 day 1 2223. Here we elucidated the role of. The indicator amino acid oxidation iaao technique provides a noninvasive estimate of the protein intake that maximizes whole body protein synthesis rates and net protein balance. Whole body net protein synthesis can be estimated by nitrogen balance. Measure the whole body protein synthesis rate. This was done by comparing the indirect picou taylor roberts method which involves measuring the urinary urea en richment at plateau with the direct garlick method using the homogenized whole rat for the garlick determination.
Gallery of Whole Body Protein Synthesis Measurement
